Genetic Alphabet Expansion

Genetic Alphabet Expansion Technology

Introducing Xenolis, where innovation meets precision. With our proprietary artificial base pairs (Ds−Px and Ds−Pa), we are crafting novel nucleic acids like XenoAptamers and more.

Step into genetic innovation’s future with our cutting-edge genetic alphabet expansion technology! By harnessing artificially created third base pairs (unnatural base pairs), we are transforming traditional 4-letter DNA into groundbreaking 6-letter DNA. Through this revolutionary Central Dogma approach, we are expanding the vocabulary of nucleic acids and proteins, unlocking the potential for creating novel biomolecules and pioneering breakthroughs in a wide area, including diagnostics, drug development, DNA data storage, and creation of biopolymers and organisms with novel functionality. Xenolis provides T7 transcription reagents and custom template DNA synthesis for site-specific modification of RNA molecules.

References

Front. Mol. Biosci., 2022., Chem. Soc. Rev., 2020., Curr. Opin. Chem. Biol., 2018., Curr. Opin. Biotechnol., 2018., J. Am. Chem. Soc., 2018., Org. Biomol. Chem., 2011., J. Am. Chem. Soc., 2010.

T7 Transcription for Site-Specific Modifications of RNA

Xenolis offers reagent kits for the site-specific introduction of various functional components into RNA through transcription. Utilizing the T7 RNA polymerase transcription system, featuring unnatural base substrates (modified PaTP) and Ds-containing DNA templates, enables precise labeling, immobilization, and introduction of diverse functional components into long-chain RNA.

References

T7 transcription:

Nature Commun., 2023.
Chem. Comm., 2017. Nucleic Acids Res., 2015.
Israel J. Chem., 2013. Chem. Comm., 2012.
Molecules, 2012. Nature Protocols, 2010.

Spinach RNA:

ACS Sensors, 2023.
Chem. Eur. J., 2022.

Custom Template DNA Synthesis

DNA Template Preparation for T7 Transcription
Xenolis offers Ds-containing DNA fragments with sequences tailored to your specifications and Ds and Px substrates reagent (dDsTP and dPxTP) for use as templates in T7 transcription. For DNA fragments shorter than 100 nucleotides, Ds-containing sequences are chemically synthesized by the standard phosphoramidite method, employing Ds and natural base amidite reagents with a DNA synthesizer. To synthesize DNA fragments longer than 100 nucleotides that include the Ds−Px pair, you can employ various methods using chemically synthesized Ds-containing DNA fragments that we provide. These methods involve polymerase reactions using dDsTP and dPxTP with chemically synthesized Ds-containing DNA templates. Duplex DNA fragments ranging from 100 to 180 nucleotides are prepared by primer extension, while those exceeding 180 nucleotides are generated via ligation, PCR, or fusion PCR.

References

T7 transcription:

Nature Commun., 2023., Chem. Comm., 2017., Nucleic Acids Res., 2015., Israel J. Chem., 2013., Chem. Comm., 2012., Molecules, 2012., Nature Protocols, 2010.

Spinach RNA:

ACS Sensors, 2023., Chem. Eur. J., 2022.

Ligation:

Biopolymer, 2021.

PCR and sequencing:

Nucleic Acids Res., 2012., Nucleic Acids Res., 2009., Chembiochem, 2020.

Fusion PCR:

J. Nucleic Acids, 2012.
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