Products

XenoAptamers

Xenolis provides a series of high-affinity and high-specificity XenoAptamers targeting proteins for detection, diagnostics, and therapeutics. Our target proteins are produced using the HEK293 expression system and glycosylated.

Our proprietary platform allows us to generate XenoAptamers with sub-nanomolar Kd values to unlock novel discoveries and applications.

We provide various types of XenoAptamers, including XenoAptamer bodies, mini-hairpin XenoAptamers, and modified XenoAptamers, as wll as customized ExSELEX™ selection and development services.

We utilize our many years of experience and the latest high-throughput, automated technologies to deliver XenoAptamer-based solutions to the customer base for a wide range of life science applications.

Explore our new Xenolis catalogue. For more information, please contact us ↗ .

XenoAptamer List

On small screens, scroll the table horizontally.

- Length: with mini-hairpin (9-mer at the 3' terminus)

- Size: 1 / 5 / 10 nmol or Bulk Inquiry ↗ .

- Modification: unmodified, biotin (at mini-hairpin), amino-group (at mini-hairpin), or Other Modification Inquiry ↗ .

XenoAptamer Molecular Affinity Ruler

Xenolis offers a collection of XenoAptamers with diverse Kd values for the same targets. This molecular affinity ruler set is beneficial for evaluating and enhancing aptamer applicability in diagnostic device developments and drug assessments. By substituting UBs in XenoAptamers with natural bases, a set of XenoAptamers with various Kd values can be created. Explore our new Xenolis catalogue. For more information, please contact us ↗ .

Anti-VEGF165 XenoAptamer Set

Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA. ↗

Kimoto M, Shermane Lim YW, Hirao I.Nucleic Acids Res. 2019 Sep 19;47(16):8362-8374.

Anti-IFNγ XenoAptamer Set

Upcoming

Anti-IL8 XenoAptamer Set

Upcoming

DNA/RNA Engineering by Genetic Alphabet Expansion

Xenolis is offering reagents for genetic alphabet expansion systems in replication and transcription. The Ds−Px pair system can be used for replication and PCR with Ds-containing DNA templates and Ds and Px substrates (dDsTP and dPxTP) using 3’-exonuclease-proficient DNA polymerases, such as DeepVent and AccuPrime™ Pfx. The Ds−Pa pair system can be used for the site-specific incorporation of functional Pa in RNA transcripts by T7 RNA polymerase using Ds-containing DNA templates and modified PaTP, such as NH_2-, biotin-, and DFHBI-conjugated PaTPs.

We offer custom synthesis of DNA fragments containing Ds, as well as dDsTP, dPxTP, and various modified PaTPs, for the preparation of DNA templates. We are equipped to synthesize DNA oligonucleotides (up to 50-mer) at PAGE purification grade. For assistance with DNA design and non-natural transcription substrates, please contact us ↗ .

UB Replication Reagents

XTP-0100 ( d DsTP)

Size: 1 / 2 / 5 μmol

XTP-0101 (Diol- d PxTP)

Size: 1 / 2 / 5 μmol

References

Highly specific unnatural base pair systems as a third base pair for PCR amplification. ↗

Yamashige R, Kimoto M, Takezawa Y, Sato A, Mitsui T, Yokoyama S, Hirao I. Nucleic Acids Res. 2012 Mar;40(6):2793-806.

PCR amplification and transcription for site-specific labeling of large RNA molecules by a two-unnatural-base-pair system. ↗
Kimoto M, Yamashige R, Yokoyama S, Hirao I. J Nucleic Acids. 2012;2012:230943.

UB Transcription Reagents

Modified PaTP

Size: 1 / 2 / 5 μmol

References for UBP transcription protocols

Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems. ↗

Kimoto M, Hirao I. Methods Mol Biol. 2010;634:355-69.

Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcriptionfor local structural conformation analysis. ↗

Hikida Y, Kimoto M, Yokoyama S, Hirao I. Nat Protoc. 2010 Jul;5(7):1312-23.

XTP-0201 (NH_2-PaTP)

XTP-0202 (Biotin-PaTP)

XTP-0203 (DFHBI-PaTP)

Custom Synthesis of UB-containing DNAs

Xenolis offers DNA fragments containing Ds bases (up to 50 nucleotides in length) for use in transcription DNA templates through PCR or primer extension (Custom Template DNA Synthesis ↗ ).